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Large GIS raster data derived from Natural Earth Data (Cross Blended Hypso with Shaded Relief and Water)
The attached data are some large GIS raster files (GeoTIFFs) made with Natural Earth data. Natural Earth is a free vector and raster map data @ naturalearthdata.com. The data used for creating these large files was the "Cross Blended Hypso with Shaded Relief and Water". Data was concatenated to achieve larger and larger files. Internal pyramids were created, in order that the files can be opened easily in a GIS software such as QGIS or by a (future) GIS data visualisation module integrated in EnviDat. Made with Natural Earth. Free vector and raster map data @ naturalearthdata.com
Land use projections and services for Switzerland
Data and scripts of publication: Madleina Gerecke, Oskar Hagen, Janine Bolliger, Anna M. Hersperger, Felix Kienast, Bronwyn Price, Loïc Pellissier (2019) Assessing potential landscape service trade-offs driven by urbanization in Switzerland. Palgrave communications. Contains land use projections for Switzerland and scripts and data for these projections as well as the calculation of landscape services. Data Folder: Contains sub-folder with the data necessary for this study (provided were no copyright issues, otherwise placeholders with descriptions), and folders where produced data may be stored Scripts Folder: Contains scripts organized into subfolders depending on their purpose Note: Some abbreviations within the scripts and data are derived from German words and not English.
Long-term recovery of above-and belowground interactions in restored grasslands
This dataset contains all data, on which the following publication below is based. Paper Citation: _Resch, M.C., Schütz, M., Ochoa-Hueso, R., Buchmann, N., Frey, B., Graf, U., van der Putten, W.H., Zimmermann, S., Risch, A.C. (in review). Long-term recovery of above- and belowground interactions in restored grassland after topsoil removal and seed addition. Journal of Applied Ecology_ Please cite this paper together with the citation for the datafile. Study area and experimental design The study was conducted in and around two nature reserves, Eigental and Altläufe der Glatt, which were located approximately 5 km apart (47°27´ to 47°29´ N, 8°37´ to 8°32´ E, 417 to 572 m a.s.l., Canton of Zurich, Switzerland; Figure S1 and S2, Table S1). Mean annual temperature and precipitation are 9.8 ± 0.6 °C and 990 ± 168 mm (Kloten climate station 1988-2018; MeteoSchweiz, 2019). TFor this study, we used a space-for-time approach based on eight restoration sites that were between 3 and 32 years old. We measured recovery and restoration success by comparing the restored grasslands with intensively managed and semi-natural grasslands. Using a space-for-time approach requires high similarities in historical properties of the site, such as soil conditions and management regimes, to assure that temporal processes are appropriately represented by spatial patterns (Walker et al., 2010). This was the case in our study. The restored sites had similar soil conditions (i.e., soil type, structure, water availability) as the targeted semi-natural grasslands, while they shared the same agricultural legacy with intensively managed grasslands, i.e., biomass harvest and fertilization (manure and/or slurry) three to five times a year as well as tillage. We randomly established three 5 m x 5 m (25-m2) plots for plant identification and three 2 m x 2 m (4-m2) subplots for soil biotic and abiotic data collection at least 2 m away from the 25-m2 plots in each restoration site. Sites of similar age were grouped into four age classes: Y.4 (3 & 4 years after restoration), Y.18 (17 & 19 years), Y.24 (23 & 25 years), and Y.30 (27 & 32 years). Six intensively managed (Initial) and six semi-natural grassland (Target) sites complemented the experimental set-up, for a total of 36 plots. All plots were sampled under similar conditions, i.e., day of the year, air temperature, soil moisture, and time since last rain event, in June/July 2017 (intensively managed and semi-natural plots) and 2018 (restored plots). Collection of plants and selected soil biota data Plant species cover (in %) was visually estimated in each 25-m2 plot in mid-June (Braun-Blanquet, 1964; nomenclature: Lauber & Wagner, 1996). We calculated Shannon diversity and assessed plant community structure. We included soil microbial (fungi, procaryotes) and nematodes in our study as they represent the majority of soil biotic diversity and abundance (Bardgett & van der Putten, 2014), cover various trophic levels of the soil food web (Bongers & Ferris, 1999), and play key roles in soil functioning and ecosystem processes (Bardgett & van der Putten, 2014). In particular, soil nematodes were found to be well suited belowground indicators to evaluate recovery/development after restoration (e.g. Frouz, et al. 2008; Kardol et al., 2009; Resch et al., 2019). We randomly collected ten soil cores (2.2 cm diameter x 12 cm depths; sampler from Giddings Machine Company, Windsor, USA) in the 4-m2 subplots to assess soil nematode and microbial (fungal, prokaryotic) diversities and community structures. For soil nematodes, eight of the soil cores were combined and gently homogenized, placed in coolers and stored at 4 °C and transported to the laboratory (Netherlands Institute of Ecology, NIOO, Wageningen, Netherlands) within three days after collection. Free-living nematodes were extracted from 200 g of fresh soil using Oostenbrink elutriators (Oostenbrink, 1960). After extraction, each sample was divided into three subsamples, two for molecular identification and one to determine nematode abundance (see Resch et al., 2019). For the molecular work, two subsamples were stored in 70% ethanol (final volume 10 mL each) and transported to the laboratory at the Swiss Federal Research Institute WSL (Birmensdorf, Switzerland). Each subsample was reduced to roughly 200 μL by centrifugation and removal of the supernatant. The remaining ethanol was vaporized (65 °C for 3 h). Thereafter, 180 μL ATL buffer solution (Qiagen, Hilden, Germany) was immediately added and samples were stored at 4 °C until further processing. From these samples, nematode metagenomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer`s protocol, except for the incubation step which was run at 56 °C for 4 h. PCR amplification of the V6-V8 region of the eukaryotic small-subunit (18S) was performed with 7.5 μL of genomic DNA template (ca. 1 ng/μL) in 25 μL reactions containing 5 μL PCR reaction buffer, 2.5 mM MgCL2, 0.2 mM dNTPs, 0.8 μM of each primer (NemF: Sapkota & Nicolaisen, 2015; 18Sr2b: Porazinska et al., 2009), 0.5 μL BSA, and 0.25 μL GoTaq G2 Hot Start Polymerase (Promega Corporation, Madison, USA). Amplification was using an initial DNA denaturation step of 95 °C for 2 min, followed by 35 cycles at 94 °C for 40 sec, 58 °C for 40 sec, 72 °C for 1 min, and a final elongation step at 72 °C for 10 min. Filtering, dereplication, sample inference, chimera identification, and merging of paired-end reads was implemented using the DADA2 pipeline (v.1.12; Callahan et al., 2016) to finally assign amplicon sequence variants (ASVs) as taxonomic units. We combined and homogenized the remaining two soil cores to assess soil microbes, placed them in coolers (4 °C) and transported them to the laboratory at WSL. Metagenomic DNA was extracted from 8 g sieved soil (2 mm) using the DNAeasy PowerMax Soil Kit (Qiagen, Hilden, Germany) according to the manufacturer´s protocol. PCR amplification of the V3-V4 region of the small-subunit (16S) of prokaryotes (i.e., bacteria and archaea) and the ribosomal internal transcribed spacer region (ITS2) of fungi was performed with 1 ng of template DNA using PCR primers and conditions as previously described (Frey et al., 2016). PCRs were run in triplicates, pooled and sent to the Genome Quebec Innovation Centre (Montreal, QC, Canada) for barcoding using the Fluidigm Access Array technology (Fluidigm) and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, USA). Quality filtering, clustering into operational taxonomic units (OTUs, 97% similarity cutoffs) and taxonomic assignment were performed as previously described (Resch et al., 2021).Taxonomic classification of nematode, prokaryotic and fungal sequences was conducted querying against the most recent versions of PR2 (v.4.11.1; Guillou et al., 2013), SILVA (v.132; Quast et al., 2013), and UNITE (v.8; Nilsson et al., 2019) reference sequence databases. Taxonomic assignment cutoffs were set to confidence rankings ≥ 0.8 (below ranked as unclassified). Prokaryotic OTUs assigned to mitochondria or chloroplasts as well as OTUs or ASVs assigned to other than Fungi or Nematoda were manually removed prior to data analysis. The three datasets were filtered to discard singletons and doubletons. Taxonomic abundance matrices were rarefied to the lowest number of sequences per community to achieve parity of the total number of reads between samples (Prokaryotes: 10,929 reads; Fungi: 18,337 reads; Nematodes: 6,662 reads). We calculated Shannon diversity and assessed community structures for soil nematodes, prokaryotes and fungi based on their relative abundances of ASV or OTU at the taxon level. Collection of soil physical and chemical properties We randomly collected one undisturbed soil core (5 cm diameter, 12 cm depth) per 4-m2 subplot using a steel cylinder that fit into the soil corer. The cylinders were capped to avoid disturbance during transport and used to measure field capacity, rock content and fine earth density as previously described (Resch et al., 2021). We randomly collected another three soil cores (5 cm diameter, 12 cm depths) in each 4-m2 subplot to determine soil chemical properties. The cores were pooled, dried at 60 °C for 48 h and passed through a 2 mm sieve. We measured soil pH (CaCl2) on dried samples, total nitrogen (N) and organic carbon (C) concentration on dried and fine-ground samples (≤ 0.5 mm; for details see Resch et al., 2021). We calculated total N and organic C pools after correcting its concentration for soil depth, rock content and fine earth density.
Backward Trajectories
Backward trajectories were calculated from two positions: Davos Wolfgang (LON: 9.85361, LAT: 46.83551) and Weissfluhjoch (LON: 9.80646 LAT: 46.83304) for the time period February 2 until March 27 2019 using COSMO or ECMWF, respectively.
Supporting information for case study on the application of spore sampling for the monitoring of macrofungi
Resources associated with the manuscript *A case study on the application of spore sampling for the monitoring of macrofungi* ([Schlegel et al. 2024](https://doi.org/10.1111/1755-0998.13941)). Airborne environmental DNA (eDNA) monitoring of fungi was evaluated on a species-rich grassland site. Extensive fruiting body surveys yielded 29 waxcap and clavarioid species, 19 of which were also detectable in air eDNA. The analysis indicates that spores of locally occurring fruiting bodies were detected, in addition to a large diversity of common fungal species, while rare and threatened species were under-represented in the air. *Version 1* of the dataset was submitted during the review phase. *Version 2* contains minor changes and clarifications, and is up to date with the [published supplement](https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1755-0998.13941&file=men13941-sup-0001-AppendixS1.pdf) of the study. The contents of the ZIP archive: * analysis scripts in [R-Markdown](https://rmarkdown.rstudio.com) format, including the rendered PDF output with figures and tables (*analysis* directory) * eDNA sample metadata (*amplicon_meta*) and results of the amplicon clustering (*amplicon_data*) and the source code of the pipeline (*amplicon_pipeline*) * positions of all fruiting body groups found in the surveys (3838 GPS points) (*surveys*) * positions of the spore traps, taxonomy of studied grassland fungi and red-list species, and many more resources Please refer to `README.pdf` inside the archive for a complete description of the file structure and information on how to reproduce the analyses.
Environmental DNA Marine France Calanques 2022
Description: Fish environmental DNA data set collected in 2022 in the Calanques National Park The eDNA samples were collected in 2022 in two locations (Moyades, M−FPA and “Impérial du large”, I-LPA), during the winter (January- February), the summer (June to August)and fall (mid-September to November) seasons, with the sampling dates depending on the weather conditions. For the M−FPA, samples were collected between Moyades island and Riou island, while for the I-LPA, they were collected on the south side of the “Impérial du large” island. To account for the existing bathymetry, in the M−FPA the samples were taken at two sampling sites with depths of 20 and 40 m, while in the I-LPA they were taken at two sites with depths of 20 and 80 m. At each site, in-situ filtration of seawater was performed using a double-head submersible pump (Subspace, Geneva, Switzerland; nominal flow of ca. 1 L/min) strapped to an underwater scooter with 2 VigiDNA 0.20 µm filtration capsules (SPYGEN, le Bourget du Lac, France), along with disposable sterile tubing. The samples were collected along two horizontal transects (up to 400 m in length) during each closed-circuit rebreather dive, enabling the filtration of a water volume of 15 L/filter per depth, as close as possible to the substrate. Two filter replicates were collected by two divers at each sampling site, except in two cases where bad weather conditions or logistical issues meant that only one replicate was sampled. After the filtration, the remaining seawater was emptied from the capsule back on the boat and replaced by a 80 mL CL1 conservation buffer (SPYGEN, le Bourget du Lac, France). To prevent any contamination, a strict protocol was followed during the entire process, requiring disposable gloves and single-use filtration equipment. Finally, the samples were stored at room temperature. We followed a strict contamination control protocol in both field and laboratory stages. Each water sample processing included the use of disposable gloves and single-use filtration equipment to avoid any risk of contamination. Libraries were prepared with ligation using the MetaFast protocol (Fasteris). Data content: * rawdata/: contains the raw reads for each individual sample. One archive contains the paired-end reads specified by the _R1 or _R2 suffix as well as individually tagged PCR replicates (if available) together with an archive containing all extraction and PCR blank samples of the library. Reads have been demultiplexed using cutadapt but not trimmed, individual demultiplexing tags and primers remain present in the sequences. * taxadata/: contains the table with all detected taxonomy for each sample after bioinformatic processing (see Polanco et al. 2020 for details; https://doi.org/10.1002/edn3.140) and associated field metadata. * metadata/: contains two metadata files, one related to the data collected in the field for each filter, and the second related to the sequencing process in the lab (including the tag sequence, library name, and marker information for each sample)
Alpine3D simulations of future climate scenarios CH2014
Overview The CH2014-Impacts initiative is a concerted national effort to describe impacts of climate change in Switzerland quantitatively, drawing on the scientific resources available in Switzerland today. The initiative links the recently developed Swiss Climate Change Scenarios CH2011 with an evolving base of quantitative impact models. The use of a common climate data set across disciplines and research groups sets a high standard of consistency and comparability of results. Impact studies explore the wide range of climatic changes in temperature and precipitation projected in CH2011 for the 21st century, which vary with the assumed global level of greenhouse gases, the time horizon, the underlying climate model, and the geographical region within Switzerland. The differences among climate projections are considered using three greenhouse gas scenarios, three future time periods in the 21st century, and three climate uncertainty levels (Figure 1). Impacts are shown with respect to the reference period 1980-2009 of CH2011, and add to any impacts that have already emerged as a result of earlier climate change. Experimental Setup Future snow cover changes are simulated with the physics-based model Alpine3D (Lehning et al., 2006). It is applied to two regions: The canton of Graubünden and the Aare catchment. These domains are modeled with a Digital Elevation Model (DEM) with a resolution of 200 m × 200 m. This defines the simulation grid that has to be filled with land cover data and downscaled meteorological input data for each cell for the time period of interest at hourly resolution. The reference data set consists of automatic weather station data. All meteorological input parameters are spatially interpolated to the simulation grid. The reference period comprises only thirteen years (1999–2012), because the number of available high elevation weather stations for earlier times is not sufficient to achieve unbiased distribution of the observations with elevation. The model uses projected temperature and precipitation changes for all greenhouse gas scenarios (A1B, A2, and RCP3PD) and CH2011 time periods (2035, 2060, and 2085). Data Snow cover changes are projected to be relatively small in the near term (2035) (Figure 5.1 top), in particular at higher elevations above 2000 m asl. As shown by Bavay et al. (2013) the spread in projected snow cover for this period is greater between different climate model chains (Chapter 3) than between the reference period and the model chain exhibiting the most moderate change. In the 2085 period much larger changes with the potential to fundamentally transform the snow dominated alpine area become apparent (Figure 5.1 bottom). These changes include a shortening of the snow season by 5–9 weeks for the A1B scenario. This is roughly equivalent to an elevation shift of 400–800 m. The slight increase of winter precipitation and therefore snow fall projected in the CH2011 scenarios (with high associated uncertainty) can no longer compensate for the effect of increasing winter temperatures even at high elevations. In terms of Snow Water Equivalents (SWE), the projected reduction is up to two thirds toward the end of the century (2085). A continuous snow cover will be restricted to a shorter time period and/or to regions at increasingly high elevation. In Bern, for example, the number of days per year with at least 5 cm snow depth will decrease by 90% from now 20 days to only 2 days on average.
Groundwater time series Studibach (Rinderer et al., 2019, WRR)
Groundwater time series between 2010 and 2014 of the distributed monitoring system in the Studibach (C7), Alptal, Switzerland. Data published in Rinderer M., van Meerveld I, McGlynn B. (2019): From points to patterns – Assessing runoff source area dynamics and hydrological connectivity using time series clustering. Water Resources Research, doi: 2018WR023886R
FSM2trans snowpack simulations with HICAR input
DESCRIPTION The dataset is used to run snowpack simulations with various forcing data at different resolutions with the Flexible Snow Model (FSM2oshd). A dynamical downscaling model (HICAR) and a semi-statistical downscaling approach (COSD) are used to downscale COSMO data to resolutions of 250 m, 100 m and 50 m. Simulations are run with the operational snow cover model and a model extension including wind- and gravity-induced snow redistribution (FSM2trans). Input Data This paragraph describes the input data used to run simulations with FSM2trans HICAR The HICAR input data is created by the High-resolution Intermediate Complexity Atmospheric Research model. The input data is available for different resolutions (250m, 100m and 50m) for a domain covering complex terrain in the Swiss Alps (Dischma) COSD The COSD data was statistically downscaled within the OSHD framework to different resolutions (250m, 100m, 50m) for a domain covering complex terrain in the Swiss Alps (Dischma) Simulation files For the Simulations, a landuse file and a namelist file with the parametrizations need to be supplied to the model simulation output This simulation output that is used for the analysis of the study is provided.
Photogrammetric Drone Data Wolfgang Arelen
We conducted four drone flights in Davos Wolfgang Arelen, in 2020/21 and 2022 to obtain data for the generation of DSMs and orthomosaics at a high resolution. The data was processed with the Agisoft Metashape Professional Software.